| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Glogowski, Amelia C (11)
|
NITWST |
35.79 |
33.00 |
-2.79 |
| 2 |
Mutty, Vivian M (11)
|
NIEAST |
39.68 |
34.00 |
-5.68 |
| 3 |
Drozdowski, Jessica L (11)
|
NITWST |
36.90 |
35.66 |
-1.24 |
| 4 |
Musterait, Mallory E (11)
|
NITWST |
39.12 |
37.25 |
-1.87 |
| 5 |
Latona, Dena M (12)
|
NIHAMB |
37.19 |
38.97 |
1.78 |
| 6 |
Daley, Lily K (11)
|
NITWST |
43.62 |
40.26 |
-3.36 |
| 7 |
LeBaron, Julia L (11)
|
NIHHSC |
45.64 |
40.47 |
-5.17 |
| 8 |
Blair, Gianna M (11)
|
NITTAC |
|
42.21 |
- |
| 9 |
Wittmeyer, Paiden E (11)
|
NIHHSC |
44.03 |
45.24 |
1.21 |
| 10 |
Jones, Sophia M (11)
|
NIPVSC |
56.94 |
46.78 |
-10.16 |
| 11 |
Wdowiasz, Gracie J (11)
|
NIHHSC |
57.17 |
50.26 |
-6.91 |